Molecular Microbiology

Microorganisms exist freely or in association with other organisms, contributing significantly to biogeochemical cycles and producing secondary metabolites with biomedical applications. Some bacteria are also pathogenic to humans. To harness their biotechnological potential, isolation and identification are essential, requiring both theoretical knowledge and practical skills. We offer a technical program focused on environmental microorganism isolation, purification, and identification through marker gene sequencing. Molecular pathogen identification is a highly sought-after skill for postgraduate students pursuing careers in research, biotechnology, food processing, aquaculture, and water-related industries.

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Major Activities

Training Programs & Work Space We provide training in microbiology and molecular biology techniques for isolating and identifying bacteria from terrestrial and aquatic ecosystems. The program includes both theoretical and practical courses covering

  • Microorganism isolation and maintenance
  • DNA extraction
  • PCR primer design and marker gene amplification
  • Microbial screening for NRPS and PKS genes
  • Agarose gel electrophoresis
  • PCR product purification
  • Gene cloning and sequencing
  • Sanger sequencing PCR
  • Sequence data analysis and phylogenetic tree creation
  • NCBI data submission

Lab space and technical guidance are available for researchers conducting their own assays.

Technical Services

  • Microbial community structure analysis using 16S amplicon sequencing (NGS)
  • Microbe isolation and preservation from various environments
  • Screening bacteria for biotechnological applications (enzymes, pigments, bioremediation)
  • Microbial identification through marker gene sequencing
  • Molecular screening for bioactive potential via NRPS and PKS genes
  • Gene cloning